BACKGROUND: While physical exercise is beneficial for back and neck-shoulder pain, only few intervention studies have evaluated effects on pain in multiple body regions. Furthermore, direct measurement of pain threshold can provide additional information to self-reported pain intensity. OBJECTIVES: To evaluate the effect of workplace versus home-based physical exercise on pressure pain threshold (PPT) and musculoskeletal pain intensity in multiple body regions. STUDY DESIGN: Secondary analysis of an examiner-blinded, cluster randomized controlled trial with allocation concealment. METHOD: Two-hundred female healthcare workers from 18 departments at three hospitals were cluster-randomized to 10 weeks of: (1) home-based physical exercise (HOME) performed alone during leisure time for 5x10min per week or (2) workplace physical exercise (WORK) performed in groups during working hours for 5x10min per week and up to 5 motivational coaching sessions. PPT (neck, lower back, lower leg) and perceived pain intensity in multiple body regions (feet, knee, hips, lower and upper back, elbow, hand, shoulder, neck, and head) were measured at baseline and 10-week follow-up. RESULTS: In some of the body regions, PPT and pain intensity improved more following WORK than HOME. Between-group differences at follow-up (WORK versus HOME) were 41 kPA (95% CI 13 to 70, effect size (ES) 0.22) for PPT in the lower back, and -0.7 (95% CI -1.0 to 0.3, ES 0.26) and -0.6 points (95% CI -0.9 to -0.2, ES 0.23) for pain intensity in the lower back and feet, respectively. HOME did not improve more than WORK for any of the measurements. CONCLUSION: Physical exercise recommendations for healthcare workers should consider the setting, ie, performing supervised group-based exercise at work and motivational coaching sessions is more effective than exercising alone at home.

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