OBJECTIVE: The present systematic review aimed to investigate the impact of school-based physical activity (PA) interventions of "active breaks" on PA levels, classroom behaviour, cognitive functions, and well-being in pre-adolescents and adolescents attending secondary and high school. METHODS: In March 2021, we performed a systematic research in CINAHL, Cochrane Library, Embase, Medline, and PsycINFO databases and through grey literature. Quality assessment was performed in accordance with the Cochrane tool for quality assessment for RCTs and the STROBE tool for observational studies. We included studies that investigated classroom PA interventions led by teachers such as active breaks or physically active lessons on PA levels, classroom behaviour, cognitive function, and quality of life in pre-adolescent and adolescent population attending secondary and high school. SYNTHESIS: Three studies met the inclusion criteria. Two studies showed a positive effect of active breaks on students' classroom behaviour and quality of life. One study registered a positive effect in the increase in school PA levels; unfortunately, this effect was not found in the overall levels of PA or in the reduction of sedentary behaviour. All three studies showed the feasibility and acceptability of active breaks intervention in secondary and high school settings. CONCLUSION: This systematic review suggests the potential benefit of this type of intervention integrated in the secondary and high school curriculum on classroom behaviour, school PA levels, and well-being.

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