OBJECTIVES: One of the most important social developments of the 21st century is older people struggling with problems such as muscle mass loss and vitamin D deficiency. The present study investigated the effect of combined exercises with and without blood flow restriction and vitamin D supplementation on serum alkaline phosphatase (ALP) levels in older women. METHODS AND MATERIALS: This study was conducted as a clinical trial on 40 older women in three intervention groups and a control group in the spring of 2021. The people in the intervention group were subjected to the exercise program for eight weeks, three sessions per week. Combined exercises included aerobic plus resistance exercises (with an intensity of 80% 1 maximum repetition) and combined exercises with blood flow restriction (20%-30% 1 maximum repetition). In the supplement group, a 50000 mg vitamin D capsule was used once a week. ALP indices vitamin D supplement amount was measured in two stages before and after the test. Data analysis was done using analysis of covariance and paired t-test in SPSS software at a significance level of alpha = 0.05. RESULTS: The present study showed that vitamin D supplementation had no significant effect on ALP levels (p > 0.05). While combined exercises with and without blood flow restriction significantly affected the target index (p < 0.05). CONCLUSION: It seems that combined exercises with and without blood flow restriction have a positive effect on the level of ALP in older women, while taking vitamin D supplements alone in the short term cannot have beneficial effects on the level of ALP in older women.

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